Whole transcriptome profiling with RNA sequencing measures gene and transcript abundance, and identifies known and novel features of the transcriptome, at a given time. Through interrogation of the whole transcriptome, researchers can determine global expression levels of coding and non-coding transcripts and identify exons, introns and their junctions. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.
~90% of total RNA is abundant ribosomal RNA noise
The high abundance of ribosomal sequences in samples often results in more background noise than usable data. Detecting novel transcripts or low-expressing transcripts becomes a time-consuming challenge, where the biologically relevant data you need is often lost in the clutter.
Traditional methods exist to remove ribosomal RNA, but these methods tend to be expensive and time consuming. They also usually require large amounts of RNA input because depletion must be done before NGS library construction begins. CRISPRclean differs from traditional methods in that depletion happens after library adapters are ligated. This allows for a more complex library with clearer results - and significantly less RNA input.
With CRISPRclean, you can access an unbiased whole transcriptome – without the noise
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For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/22/2a/ef/6657ca336c9c08/US10604802.pdf
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/a7/54/96/52ed494ab92016/EP3102722B1.pdf