CRISPRclean® technology improves single cell transcriptional profiling, enabling scientists to discover and see signals that were previously obscured by abundant genomic sequences
SAN DIEGO, Calif. – March 29, 2022 – Jumpcode Genomics, a genome technology platform company focused on improving the understanding of human biology, today announced the launch of the CRISPRclean Single Cell RNA Boost Kit. The method leverages Jumpcode’s patented CRISPR-based technology to remove uninformative sequences from libraries, enhancing single cell experiments and enabling scientists to extract greater insights from genomic data. The technology has broad applicability across basic and translational research, clinical applications, infectious disease and oncology.
Single cell studies require a vast amount of sequencing – anywhere from 25,000 to 150,000 reads per cell – in order to understand transcript levels within individual cells. Such data sets are traditionally filtered and normalized prior to downstream interpretation. Instead of removing those reads in-silico, Jumpcode’s CRISPRclean technology depletes the molecules generating those reads in-vitro ahead of sequencing. This redistributes 50% of sequencing reads to unique biologically relevant transcripts – boosting usable data for researchers.
“As we’ve seen incredible growth in single cell analysis, it’s become evident that RNA sequencing can help fuel the discovery of rare cell types and development of new drug targets. However, historically, there have been many challenges in realizing the full potential of single cell RNA sequencing, including the presence of abundant uninformative transcripts that can mask impactful biological findings,” said Yaron Hakak, Ph.D., CEO of Jumpcode Genomics. “Jumpcode’s new CRISPRclean Single Cell RNA Boost Kit enables unbiased discovery by addressing this needle in a haystack problem, allowing scientists to see signals that have been previously obscured.”
Benefits of the Jumpcode CRISPRclean Single Cell RNA Boost Kit include:
- The ability to double informative transcriptomic reads while enhancing cell type identification
- Boost usable data by reallocating 50% of sequencing reads from uninformative sequences to transcripts used for differential analysis
- Gain a deeper view of expression profiles of individual cells and increase sensitivity to detect rare cell populations
To learn more about the CRISPRclean Single Cell RNA Boost Kit, click here.
About Jumpcode Genomics
Jumpcode Genomics is changing genomics with technology that allows scientists to break barriers limiting their research. Combining CRISPR-based technology and next-generation sequencing it’s now possible to search for and find novel signals that were previously undetectable. Our technology removes uninformative sequences, allows researchers to extract greater insights, and broadens the understanding of human biology in fields from research to clinical applications. Discover what we can do.