Banner image of hundreds of different-colored cells

What We Do

Boost sensitivity in genomics by removing undesired fragments prior to sequencing with DepleteX® 

Bulk RNA-Seq

Abundant ribosomal, mitochondrial, globin, insulin and housekeeping genes

Single Cell Sequencing

Ribosomal, mitochondrial, and non-variable genes

DNA Sequencing

Repetitive DNA, contaminating human DNA in microbial samples

The massive throughput increase of sequencing technology has powered a new era of genomics. Unfortunately, sequencing reads are wasted on abundant and uninformative sequences that limit the ability to detect lower abundance signals.  This is the classic ‘needle in a haystack’ problem of genomics.  Our solution?  DepleteX technology removes fragments prior to sequencing and removes the haystack to better see all the needles in a sample. 

DepleteX technology harnesses the specificity of CRISPR-Cas9 to degrade abundant, uninformative sequences in next-generation sequencing (NGS) libraries. Sequencing reads are then spent on fragments of interest so you can better see low level gene expression, boost somatic mutation detection, or better detect pathogens in metagenomic samples. 

Now you can see exactly what you want, breaking through the clutter—and breaking new ground. 

Benefits of DepleteX technology

Highly programmable

Increases sensitivity

Simple automatable workflow

Genomic analysis
platform agnostic

How DepleteX works

DepleteX mediated depletion can be integrated directly into NGS library prep protocols at various points depending on the application. CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs. After targeted sequences are cut, they cannot be PCR amplified and are excluded from a sequencing library.

Diagram showing how CRISPRclean works

Applications

DepleteX technology is a scalable and specific removal method for unwanted sequences for many applications.
View the applications below to learn how Jumpcode is driving discovery today.

Single Cell Analysis

Gain a deeper view of expression profiles of individual cells

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Microbiome

Characterize microbial populations and their impact on human health

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Infectious Disease Surveillance

Detect known and novel pathogens to accelerate outbreak investigations

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Whole Transcriptome Profiling

Measure gene and transcript activity to interrogate gene expression patterns

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Customer Story – ImYoo

Isolating informative RNA gives ImYoo™ greater power to grow their business.

ImYoo is a single-cell transcriptomics startup that helps biopharma companies stratify study participants to optimize clinical trials. The company is using single-cell gene expression profiling to understand immune variability and personalize information.

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For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.

For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.