Single Cell Analysis
Gain a deeper view of single cell expression profiles with CRISPRclean.
Transcriptional profiling has been revolutionized with high throughput, massively parallel, single cell RNA sequencing. Microfluidic technologies produce comprehensive data sets that allow investigators to understand complex cell mixtures, identify cell types present in healthy and diseased tissues, and create cell type specific transcriptional signatures. These technologies are dramatically enhancing our ability to identify transcriptional and cellular perturbations driving disease and basic biology understanding at the individual cell level.
Uninformative transcripts dominate sequencing reads.
One problem faced by single cell RNA-seq methods is that greater than 90% of single cell RNA-seq dataset is noise and uninformative1. While computational algorithms have evolved to parse out the true signal, commonly available microfluidic technologies enable only sparse sampling of RNAs from each cell, with many genes represented by a few sequencing reads. This limit is partially due to an abundance of biologically uninformative RNAs which dominate sequencing reads and limit detection of the moderately expressed transcripts that often drive biological differences between cell types.
What if there was a simple solution that removes uninformative reads in-vitro, thereby redistributing sequencing reads to unique, biologically relevant transcripts?
Depletion identifies two additional clusters with biologically relevant lowly expressed markers in spatial transcriptomics ovarian tissues.
~52% of reads within ovarian tumor was redistributed after depletion using CRISPRclean. This nearly doubles the percent of reads aligning to the transcriptome from 40% to 70%. Depletion increased statistical confidence in identifying spatial, biologically relevant genes.
Jumpcode-depleted PacBio MAS-Seq data identifies four additional rare cell clusters in PBMC
Depleted samples have 4 additional clusters under the same analytical conditions as the control because of the increased detection of highly variable genes (HVGs).
Explore featured resources
DepleteX™ Single Cell RNA Boost Kit
Increase scRNA-seq coverage of low-expression genes and isoforms with depletion
Identify more genes per UMI in ovarian tissues for spatial gene expression
Customer Story – ImYoo
Isolating informative RNA gives ImYoo™ greater power to grow their business.
ImYoo is a single-cell transcriptomics startup that helps biopharma companies stratify
study participants to optimize clinical trials. The company is using single-cell gene
expression profiling to understand immune variability and personalize information.
Connect with us to learn more
1 Qiu, P. Embracing the dropouts in single-cell RNA-seq analysis. Nat Commun 11, 1169 (2020).
For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.