CRISPRclean Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)
Specific, unbiased rRNA depletion and library prep for RNA-Seq.
RNA samples frequently contain up to 90% ribosomal RNA. Transcriptome sequencing without removing these overabundant rRNA sequences reduces sequencing capacity and makes it harder to detect lower expressing, biologically relevant transcripts. See how CRISPRclean can help your research with effective rRNA removal.
Highlights
- Effective single tube, multi-species depletion with CRISPR-Cas9 mediated technology
- Increases sensitivity to detect lower expressing, biologically relevant transcripts
- Optimized library prep and depletion workflow generates high quality representation of transcripts
- Consistent full-length, uniform transcript coverage, and high library complexity
- One day workflow: 7.5 hours assay with 3 hours of hands-on time
Specification | |
Catalog | KIT1014 |
---|---|
Samples per kit | 24 |
Assay time | 7.5 hours |
Hands-on time | 3 hours |
Sample types | Total RNA |
Input quantity | 5 to 100 ng |
Strand specificity | >98% Directional and strand specific |
Compatible species | Human, mouse, and rat |
Designed to deplete | Human 5S, 5.8S, 18S and 28S rRNA genes, 45S rRNA precursor, mitochondrial 12S and 16S rRNA genes |
Multiplex | Up to 96 unique dual index adapter barcodes. Requires KIT1017 CRISPRclean Unique Dual Index Adapter Plate for RNA Prep (Set A) |
One-day workflow from RNA to sequencing-ready library
Specific and unbiased
CRISPRclean Stranded Total RNA Prep with rRNA Depletion produces extremely low library bias. ERCC reads counts were highly correlated, between depleted (y-axis) and undepleted libraries (x-axis), indicating the CRISPRclean rRNA depletion method is highly specific, unbiased, and accurate. This allows for gene expression measurements to be more accurately represented than those that would be obtained from an undepleted sample. Illumina RiboZero Plus Depletion paired with Jumpcode’s stranded RNA prep shows lower ERCC read count correlation and higher bias.
CRISPRclean Depletion |
RiboZero Plus Depletion |
Performance
Effective single tube, multi-species depletion
Optimized library prep and depletion workflow
High quality representation of human gene expression
Consistent full-length, uniform transcript coverage
Increases sensitivity to detect lower-expressing, biologically relevant transcripts
CRISPRclean Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)
24 samples
$2,160 Add to CartResources
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For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.