User Manuals
Single Cell
| User Manual Name (click to download) | Description | Sequencing Technology | Species | Product Catalog Number |
|---|---|---|---|---|
| Single Cell RNA Boost Kit with panels | Describes how to remove ~50% uninformative human reads that are not used in downstream analysis and redistribute the reads to the transcriptome from 10x Chromium Next-GEM Single Cell libraries. | Short read | Human | KIT1018, KIT1022, KIT1024, KIT1025 |
| Single Cell RNA Boost Kit with PacBio MAS-Seq | Describes how to use Single Cell RNA Boost to deplete the single-cell cDNA material generated from the 10x Chromium Next GEM Single Cell 3’ Kit v3.1 in order to prepare inputs for the PacBio MAS-Seq for 10x Single Cell 3′ Kit and PacBio sequencing. | PacBio Sequel II/Iie and Revio systems | Human | KIT1018, KIT1022, KIT1024, KIT1025 |
Bulk RNA
RNA Decision Tree
Jumpcode depletion panels can be applied to multiple use cases. Follow this decision tree to find out which RNA depletion user manual is recommended for your study.
Download| User Manual Name (click to download) | Description | Sequencing Technology | Species | Product Catalog Number |
|---|---|---|---|---|
| Stranded total RNA prep with depletion | Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat rRNA and/or high expressing gene panels. | Short read | Human, Mouse, Rat | KIT1014, KIT1022, KIT1024, KIT1025, KIT1027 |
| Plus Stranded Total RNA Prep with Depletion | Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat, and 212 bacteria rRNA. | Short read | Human, Mouse, Rat, Bacteria | KIT1016 |
| Unique Dual Index Adapter Plate for RNA Prep (Set A) | This spreadsheet lists the P5 and P7 adapter sequences for use. | Short read | n/a | KIT1014, KIT1016, KIT1017 |
| Post-library depletion for poly A selected libraries | Describes how to remove high expressing genes from poly A selected and ribodepleted libraries to improve sequencing sensitivity and performance. | Short read | Human | KIT1020, KIT1022, KIT1024, KIT1025, KIT1027 |
| Post-library rRNA and panel depletion | Describes how to remove human, mouse, rat rRNA and/or high expressing genes from RNA-Seq libraries that has not been treated with a method to remove rRNA. | Short read | Human, Mouse, Rat | KIT1031, KIT1032, KIT1033, KIT1034 |
| Post-library multiplex ribodepletion | Describes how to remove overabundant human, mouse, rat, or bacterial-species rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale. | Short read | Human, Mouse, Rat | KIT1026, KIT3000s |