User Manuals

Single Cell

User Manual Name (click to download)	Description	Sequencing Technology	Species	Product Catalog Number
Single Cell RNA Boost Kit with panels	Describes how to remove ~50% uninformative human reads that are not used in downstream analysis and redistribute the reads to the transcriptome from 10x Chromium Next-GEM Single Cell libraries.	Short read	Human	KIT1018, KIT1022, KIT1024, KIT1025
Single Cell RNA Boost Kit with PacBio MAS-Seq	Describes how to use Single Cell RNA Boost to deplete the single-cell cDNA material generated from the 10x Chromium Next GEM Single Cell 3’ Kit v3.1 in order to prepare inputs for the PacBio MAS-Seq for 10x Single Cell 3′ Kit and PacBio sequencing.	PacBio Sequel II/Iie and Revio systems	Human	KIT1018, KIT1022, KIT1024, KIT1025

User Manual Name (click to download)	Description	Sequencing Technology	Species	Product Catalog Number
Stranded total RNA prep with depletion	Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat rRNA and/or high expressing gene panels.	Short read	Human, Mouse, Rat	KIT1014, KIT1022, KIT1024, KIT1025, KIT1027
Plus Stranded Total RNA Prep with Depletion	Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat, and 212 bacteria rRNA.	Short read	Human, Mouse, Rat, Bacteria	KIT1016
Unique Dual Index Adapter Plate for RNA Prep (Set A)	This spreadsheet lists the P5 and P7 adapter sequences for use.	Short read	n/a	KIT1014, KIT1016, KIT1017
Post-library multiplex ribodepletion	Describes how to remove overabundant human, mouse, rat, or bacterial-species rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale.	Short read	Human, Mouse, Rat	KIT1026, KIT3000s