User Manuals

Single Cell

User Manual Name (click to download)DescriptionSequencing TechnologySpeciesProduct Catalog Number
Single Cell RNA Boost Kit with panelsDescribes how to remove ~50% uninformative human reads that are not used in downstream analysis and redistribute the reads to the transcriptome from 10x Chromium Next-GEM Single Cell libraries.Short readHumanKIT1036, KIT1022, KIT1024, KIT1025
Single Cell RNA Boost Kit with PacBio MAS-SeqDescribes how to use Single Cell RNA Boost to deplete the single-cell cDNA material generated from the 10x Chromium Next GEM Single Cell 3’ Kit v3.1 in order to prepare inputs for the PacBio MAS-Seq for 10x Single Cell 3′ Kit and PacBio sequencing.PacBio Sequel II/Iie
and Revio systems
HumanKIT1036, KIT1022, KIT1024, KIT1025

Bulk RNA and DNA Depletion

User Manual Name (click to download)DescriptionSequencing TechnologySpeciesProduct Catalog Number
Stranded total RNA prep with depletionDescribes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat rRNA and/or high expressing gene panels.Short readHuman, Mouse, RatKIT1014, KIT1022, KIT1024, KIT1025, KIT1027
Plus Stranded Total RNA Prep with DepletionDescribes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat, and 212 bacteria rRNA.Short readHuman, Mouse, Rat, BacteriaKIT1016
Unique Dual Index Adapter Plate for RNA Prep (Set A)This spreadsheet lists the P5 and P7 adapter sequences for use.Short readn/aKIT1014, KIT1016, KIT1017
Post-library multiplex ribodepletion (HMR)

Describes how to remove overabundant human, mouse, rat rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale.Short readHuman, Mouse, RatKIT1026, KIT3000s
Post-library multiplex ribodepletion (HMR Plus)Describes how to remove overabundant human, mouse, rat, or bacterial-species rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale.Shot readHuman, Mouse, Rat, pan-bacterialKIT1030
High Expressing Gene Depletion Describes how to remove highly expressed RNA from whole transcriptome to improve sensitivity of low-frequency variants Short readHumanKIT1020
Mitochondrial DNA Depletion Describes how to remove mitochondrial DNA from prepared DNA sequencing libraries Short readHuman KIT2002
Nasopharyngeal RNA Depeltion Describes how to prepare strand-specific RNA libraries depleted of human and bacterial ribosomal RNA (rRNA) and abundant human nasopharyngeal mRNA for sequencing on Illumina® instrumentsShort readHumanKIT1039
Human DNA DepletionDescribes how to remove human DNA from samples with high human content and reassign sequencing reads to microbial contentShort readHumanKIT2020

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