DepleteX® Single Cell RNA Boost Kit

Boost usable single cell RNA seq data with DepleteX®

This product is a new and improved replacement for CRISPRclean Single Cell RNA Boost Kit (KIT1018).

DepleteX® removes abundant and uninformative fragments before sequencing, redistributing 50% sequencing clusters to informative reads that normally would be filtered out prior to secondary analysis.  This kit leverages Cas9 depletion with an optimized guide set to remove ribosomal and mitochondrial mRNA, non-transcriptomic reads, and highly expressed non-variable genes.  This new kit includes a separate tube for non-variable gene depletion content that can be included or excluded as needed.

DepleteX Single Cell RNA Boost Kit gives you the ability to cut through the noise and maximize usable data in your single cell RNA sequencing.

Highlights

• ~50% boost in reads useful for secondary analysis
• Validated for both short and long read sequencing
• Depletes ribosomal, mitochondrial, non-transcriptomic reads, and non-variable genes (optional) from single cell libraries
• Simple 3 step protocol

Simple 3-step protocol integrated into 10x Genomics® Chromium™ Next GEM Single Cell 3’ workflow

100% increase in reads mapped to the transcriptome with DepleteX™ Single Cell RNA Boost Kit.

1.5x improvement in detection sensitivity with depletion

Boost in detection sensitivity of genes and UMIs per cell for depleted PBMC samples compared to undepleted sample.

Gain a deeper view of expression profiles

One additional cell type was identified in PBMC samples treated with DepleteX. UMAP plots of cell clusters with and without depletion using PBMC samples showed that depletion did not perturb cell type calls.