What will you do when repeats no longer complicate genome analysis?
CRISPRclean enables researchers to remove highly repetitive fragments before sequencing that can complicate plant and animal genome analysis. Genotyping by sequencing (GBS) applications particularly benefit from this approach, in some cases achieving more than a 5x increase in useable sequences.
Plant and animal genotyping by sequencing (GBS) is a powerful tool to assess genomic variation across a large cohort. When using whole genome libraries for sequencing, a large number of reads are taken up by repetitive sequencing that do not provide useful genotyping information. This source of ‘noise’ in each sample limits the total number of samples that can be analyzed in a given experiment while maintaining appropriate level of SNP sensitivity.
Repetitive sequences no longer limit GBS sensitivity
Both plant and animal genomes contain abundant repetitive sequences that comprise a large percentage of whole genome sequencing reads. CRISPRclean removes these sequences at the library construction stage prior to sequencing so that sequencing coverage of SNP informative fragments is maximized. Jumpcode has partnered with multiple customers to optimize GBS and whole genome sequencing applications in a range of plant and animal genomes, in some cases resulting in >5-fold increase in useable reads.
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Programmable and specific CRISPR guide RNA design
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For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.