CRISPRclean Single Cell RNA Boost Kit

Double your transcriptomic reads for 10x Genomics® libraries

Traditionally, single cell data processing incorporates certain filtering and normalization steps prior to cell clustering and downstream interpretation. Instead of removing those reads in-silico, CRISPRclean removes those reads in-vitro ahead of sequencing, redistributing ~50% sequencing clusters to unique biologically relevant transcripts —allowing you to maximize gene and UMI sensitivity.

CRISPRclean leverages Cas9 depletion and a specifically designed guide set to remove reads that are filtered before secondary analysis. CRISPRclean Single Cell RNA Boost Kit gives you the ability to cut through the noise with minimal impact on your workflow, and maximum confidence in your results.

Highlights

  • Gain a deeper view of expression profiles of individual cells
  • 1.5x improvement in gene and UMI detection sensitivity
  • Boost usable data by cutting wasted sequencing by ~50%
  • Depletes sequences not used for secondary analysis including unaligned reads, ribosomal, mitochondrial, and non-variable genes

Kit includes single cell guide RNA and reagents for 24 samples.

Jumpcode validated on 10x Genomics Chromium Next GEM Single Cell 3’ Reagent Kits v3.1. (sold separately)

$6,000.00

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