DepleteX® RNA Depletion Panel (Liver)

Pinpoint low expressing transcripts with our focused RNA depletion panel for liver tissue

Our DepleteX tissue-specific RNA depletion panels remove the highest expressing transcripts in-vitro ahead of sequencing, redistributing ~50% sequencing clusters to unique biologically relevant transcripts —allowing you to maximize UMI and gene sensitivity.

Maximize sequencing coverage in liver tissue

RNA samples typically have a small number of highly expressed, well-characterized transcripts that outcompete low expressing transcripts for sequencing coverage. Resequencing these transcripts adds little biologically relevant information. The lack of sequencing coverage, coupled with significant noise from high expressing transcripts, limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers.

DepleteX leverages Cas9 depletion, and a guide set designed to remove ~50% of the highest expressing transcripts from tissues, as determined by queries from the GTEx portal. These guide sets were designed to maximize the re-assignment of sequencing reads by removing a minimal number of gene encoding transcripts—preserving relevant and novel biological transcripts. These panels were designed for RNA-Seq libraries that are already treated with ribodepletion or poly-A selection.

Highlights

• Liver panel designed to deplete ~50% of expressed transcripts in a normal liver tissue​
• Increase sensitivity by ~50% to see more biologically relevant transcript
• Maximize variant and isoform detection with less sequencing
• Integrate easily into your workflow with a simple and flexible protocol

About

• Kit contains tissue-specific guide RNA and depletion reagents for post-library depletion
• Compatible with CRISPRclean Single Cell RNA Boost (KIT1018) & CRISPRclean RNA Prep HMR (KIT1014)