DepleteX® Human DNA Depletion Kit (Early Access)

Boost microbial detection with unbiased human DNA depletion.

 

Human microbiome samples often consist of a mixture of human and microbial DNA. For some samples, contaminating human DNA can be such a large proportion of reads that the sampling of microbial fragments is significantly inhibited. Spending sequencing reads on contaminating human DNA leads to decreased microbial genome coverage, decreased microbial community resolution, and increased sequencing costs. The DepleteX Human DNA Depletion Kit utilizes a simple CRISPR-based depletion to cut unwanted human molecules that are subsequently removed by an exonuclease treatment. This kit gives you the ability to sequence more microbial reads from your metagenomic samples and get the most comprehensive and unbiased view of the microbiome.

Highlights

  • Unbiased human DNA depletion preserves microbial community
  • Improved microbial genome coverage previously obscured by human DNA
  • Increased detection sensitivity of low abundance microbial species

Specification
Catalog KIT2020
Samples per kit 24
Assay time 8 hours
Hands-on time 2.5 hours
Sample type Human-derived tissues with high human DNA contamination
Input >250 ng
Designed to deplete Human DNA fragments


Unbiased Depletion

Unbiased boost in reads across bacterial species after human DNA depletion

Boost Microbial Reads

16 fold increase in bacterial reads after human fragment depletion

 

 

DepleteX® Human DNA Depletion Kit (Early Access)

24 samples

$3,000 Add to Cart

How can we boost your research?

Schedule a Call

Resources

Download helpful product documents.

Connect with us to learn more

For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.

For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.