Human DNA Depletion Kit Early Access

Boost microbial detection with unbiased human DNA depletion.


Human microbiome samples often consist of a mixture of human and microbial DNA. For some samples, contaminating human DNA can be such a large proportion of reads that the sampling of microbial fragments is significantly inhibited. Spending sequencing reads on contaminating human DNA leads to decreased microbial genome coverage, decreased microbial community resolution, and increased sequencing costs. The Human DNA Depletion Kit utilizes a simple CRISPR-based depletion to cut unwanted human molecules that are subsequently removed by an exonuclease treatment. This kit gives you the ability to sequence more microbial reads from your metagenomic samples and get the most comprehensive and unbiased view of the microbiome.

Be among the first to use this early access kit.



  • Unbiased view of the human microbiome
  • Specific CRISPR-mediated depletion of human DNA
  • Improved microbial genome coverage previously obscured by human DNA
  • Increased detection sensitivity of low abundant microbial species

Catalog KIT2000
Samples per kit 24
Assay time 8 hours
Hands-on time 2.5 hours
Sample type Human-derived tissues with high human DNA contamination
Input 50 ng – 1 ug
Input DNA size It is recommended that prior to starting the depletion protocol to use size selection for larger fragments
Designed to deplete Human DNA fragments
Jumpcode validated DNA library prep Illumina DNA Prep, Tagmentation

Unbiased human DNA depletion in contrived samples

chart depicting unbiased human dna depletion contrived samples

Boost microbial coverage with less sequencing

Significant increase in breadth of coverage for S. aureus at 10M reads after depletion for contrived samples spiked-in with Zymo community control at 99% human and 1% microbial.

Depletion of human DNA results in read allocation to microbial sequences in clinical vaginal sample.

>10-fold change in reads aligning to microbial sequences found in contrived samples spiked-in with Zymo controls (99% human and 1% microbial).

Improved species detection

Depletion increases the number of species detected from 10 to 46 species in clinical vaginal sample. The original 10 species found in untreated is also found in the depleted clinical vaginal sample.

One day workflow from DNA to library prep ready

8 hours total assay time with 2.5 hours of hands-on time and 2 safe stopping points.

Human DNA Depletion Kit Early Access

$3,000 Add to Cart

Get early access to Human DNA Depletion today

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Be among the first to test early access kits

For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available here.

For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available here.