Harness the specificity of CRISPR to refine your NGS library
What will your samples tell you?
Your samples are full of fascinating potential—discoveries waiting to be found. Sometimes though, that information gets left behind. Lost in the clutter of sequences. Caught up in the lines of data. It’s why we created CRISPRclean.
CRISPRclean technology harnesses the specificity of CRISPR-Cas9 to degrade abundant, uninformative sequences in prepared next-generation sequencing (NGS) libraries. This method, which can be completed in 4 hours, is seamlessly integrated into NGS workflows by adding Cas9/RNA complexes to the DNA of prepared libraries.
Now you can see exactly what you want, breaking through the clutter—and breaking new ground.
How CRISPRclean works
CRISPRclean mediated depletion fits easily into NGS workflows, using prepared NGS library as input. CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs, and the complexes are mixed with the library DNA. After the targeted sequences are cut, they are not included in subsequent sequencing. The refined library (non-targeted sequences) is size-selected using magnetic beads, and then amplified. The output is a refined, sequence-ready NGS library. The entire process can be completed in 4 hours - saving valuable time while producing clear, accurate results.
Get started with CRISPRclean.
For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/22/2a/ef/6657ca336c9c08/US10604802.pdf
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/a7/54/96/52ed494ab92016/EP3102722B1.pdf