CRISPRclean Technology

Harness the specificity of CRISPR to refine your NGS library

What will your samples tell you? Your samples are full of fascinating potential—discoveries waiting to be found. Sometimes though, that information gets left behind. Lost in the clutter of sequences. Caught up in the lines of data. It’s why we created CRISPRclean™.

CRISPRclean technology harnesses the specificity of CRISPR-Cas9 to degrade abundant, uninformative sequences in prepared next-generation sequencing (NGS) libraries. With CRISPRclean, you can see more lower expressing transcripts and shift your sequencing power for deeper coverage and improved signal.

Now you can see exactly what you want, breaking through the clutter—and breaking new ground.

How CRISPRclean works

CRISPRclean mediated depletion is integrated into a stranded total RNA sequencing library prep protocol. After the adapter ligation step, CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs. After targeted sequences are cut, they cannot be substrates for PCR amplification and subsequent sequencing. The refined libraries (non-targeted sequences) is size-selected using magnetic beads. The output is a refined, sequence-ready NGS library.


  • Highly programmable
  • Achieves efficient depletion
  • Increased sensitivity
  • Simple automatable workflow

For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning.  The patent publication is available at

For the EP patent, the patent number is EP3102722 entitled Genome Fractioning.  The patent publication is available at

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