CRISPRclean Technology

Harness the specificity of CRISPR to enrich your NGS library

What will your samples tell you?

Your samples are full of fascinating potential—discoveries waiting to be found. Sometimes though, that information gets left behind. Lost in the clutter of sequences. Caught up in the lines of data. It’s why we created CRISPRclean. 

CRISPRclean technology harnesses the specificity of CRISPR-Cas9 to degrade abundant, uninformative sequences in prepared next-generation sequencing (NGS) libraries. This method, which can be completed in 4 hours, is seamlessly integrated into NGS workflows by adding Cas9/RNA complexes to the DNA of prepared libraries.

Now you can see exactly what you want, breaking through the clutter—and breaking through new ground.

How CRISPRclean works

CRISPRclean depletion uses a prepared next-generation sequencing library as input. CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs, and the complexes are mixed with the library DNA. After the targeted sequences are cut, they cannot be substrates for PCR amplification and subsequent sequencing. The enriched library (non-targeted sequences) is size-selected using magnetic beads, and then amplified a small number of rounds. The output is an enriched, sequence-ready NGS library.

CRISPRclean Workflow

CRISPRclean mediated depletion fits seamlessly into NGS workflows. CRISPRclean depletion uses prepared NGS library as input. CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs, and the complexes are mixed with the library DNA. After the targeted sequences are cut, they cannot be substrates for PCR amplification and subsequent sequencing. The enriched library (non-targeted sequences) is size-selected using magnetic beads, and then amplified a small number of rounds.  The output is an enriched, sequence-ready NGS library. The entire CRISPRclean workflow can be completed in four hours.

Decide. Detect. Discover

  • Highly programmable
  • Achieves efficient depletion
  • Increased sensitivity
  • Simple 4-hour workflow
  • Works well with low input/single cell amounts
  • Independent of library prep technologies and sequencing platforms
  • Increased throughput with simplified workflows

Get started with CRISPRclean.

Learn more about our CRISPRclean technology. Coming soon.

Discover what applications you can use with CRISPRclean. Coming soon.

Explore more products from Jumpcode. Learn more

For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning.  The patent publication is available at https://patentimages.storage.googleapis.com/22/2a/ef/6657ca336c9c08/US10604802.pdf

For the EP patent, the patent number is EP3102722 entitled Genome Fractioning.  The patent publication is available at https://patentimages.storage.googleapis.com/a7/54/96/52ed494ab92016/EP3102722B1.pdf

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