CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria)
CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit for 24 samples is an optimized directional RNA library prep and CRISPR-powered depletion workflow that increases sensitivity to detect lower expressing and biologically relevant transcripts from complex samples such as nasopharyngeal swab. An optimized workflow with 9-hour total assay time and 3.5 hours of hands-on time that is made up of 7 simple steps with multiple safe stopping points.
Powered by CRISPR-Cas9, ribodepletion is highly efficient and programmed to cut ribosomal RNA from human sequences (5S, 5.8S, 18S, 28S, 45S (precursor), mitochondrial 12S and 16S sequences), and 212 bacteria from all phyla (5S, 16S, and 23S rRNA sequences).
Improve your discovery of high-value and biologically interesting transcripts with CRISPRclean Plus.
- Ideal for analysis of samples containing complex mixtures of eukaryotic and bacterial rRNA
- 3 to 7-fold increase in coverage of SARS-CoV-2 and other genomes in nasopharyngeal (NSP) samples
- Single workflow to detect viral genomic data, microbiome composition, co-infections, and host gene expression
- Streamlined CRISPRclean workflow: stranded total RNA library prep with ribodepletion
Kit includes stranded total RNA library prep and depletion reagents for 24 RNA-Seq libraries. AMPure XP beads and KIT1017 CRISPRclean Unique Dual Index Adapter Plate for RNA Prep are sold separately and required for this protocol.